Two simple and sensitive indirect spectrophotometric methods have been developed for the estimation of Lamivudine in both pure and tablet dosage form. Methods A and B are based the formation of ion-pair complexes of the drug with dyes such as orange II and alizarin red S in acidic medium followed by their extraction with chloroform to give colored chromogen with absorption maxima at 475 nm and 445 nm respectively. Beer’s law is valid in the concentration range of 4-20 µg/ml for method A and 2-10 µg/ml for method B. These methods were validated for precision, accuracy, ruggedness and robustness. Statistical analysis proves that the methods are reproducible and selective for the estimation of the said drug in pure and tablet dosage form.
Loading....